Housekeeping Gene Selection Advisory: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and β-Actin Are Targets of miR-644a
نویسندگان
چکیده
Results of overexpression or downregulation of a microRNA (miRNA) on its target mRNA expression are often validated by reverse-transcription and quantitative PCR analysis using an appropriate housekeeping gene as an internal control. The possible direct or indirect effects of a miRNA on the expression of housekeeping genes are often overlooked. Among many housekeeping genes, expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin have been used extensively for normalization of gene expression data. Here, we show that GAPDH and β-actin are direct targets of miR-644a. Our data demonstrate the unsuitability of GAPDH and β-actin as internal controls in miR-644a functional studies and emphasize the need to carefully consider the choice of a reference gene in miRNA experiments.
منابع مشابه
Experimental verification of microRNA targets is essential, prediction alone is insufficient.
Dear Sir, We refer to recent ‘Letter-to-the-Editor’ from Ma N and Gao X regarding the use of β-actin, a predicted target of hsa-miR-145, as housekeeping control in our earlier published article (1). The authors suggested four target prediction tools, namely TargetScan, miRanda, PicTar and miRGen, could identify β-actin as a potential target of hsa-miR-145 and hence questioned its suitability as...
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